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One of the most well-characterized cyclotides, kalata B1, was initially isolated from Oldenlandia affinis and has since been identified in several Viola species, including Viola tricolor , Viola yedoensis , Viola philippica , Viola baoshanensis , and Viola odorata

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Most studies report that in humans, NCs disappear when they are about 710 years of age, whereas the age of onset of disc degeneration is 3050 years [8]

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