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Some common methods used for gene manipulation and their applications in Drosophila research are as follows: Mutant alleles LOF mutant alleles can be induced through various techniques, including chemical mutagenesis, radiation-induced mutagenesis, or more precise methodologies like CRISPR/Cas9 genome editing (Obe et al., 1971

BMJ (Clinical research ed)

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of transferred embryos 1 1.0 1.0 2 0.86 (0.54, 1.38) 0.5306 0.79 (0.46, 1.38) 0.4119 Vitamin B12 Low 1.0 1.0 Average 1.44 (0.85, 2.44) 0.1790 1.83 (1.03, 3.25) 0.0409 High 0.88 (0.53, 1.48) 0.6396 1.05 (0.60, 1.83) 0.8600 Fig