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Animal LD50 studies showed toxic levels around 320 mg/kg body weight, translating to approximately 22,400 mg for a 70 kg human

This produces 1 mg doses at 6 units on 100-unit insulin syringe (0.06 mL) and 2 mg doses at 12 units (0.12 mL)

Here's what we've learned through years in the field: Refrigeration, Not Freezing: For short-term storage (days to a few weeks), reconstituted GHK-Cu should be kept refrigerated at 2C to 8C

This collagen stimulation extends beyond simple quantity GHK-Cu also promotes production of dermatan sulfate, chondroitin sulfate, decorin, elastin, and glycosaminoglycans, all of which are critical structural components of the extracellular matrix

Nerve and Vascular Regrowth Research A smaller but mechanistically interesting cluster of studies examines GHK-Cu in nerve-regeneration and angiogenesis contexts