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Genome-wide analysis of nucleotide-level variation in commonly used Saccharomyces cerevisiae strains

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For the reverse transfection on a Freedom EVO 200 workstation (Tecan), 5 l of 250 nM siRNA per well, diluted in Opti-MEM (Thermo Fisher Scientific), were placed into a 384-well PhenoPlate (PerkinElmer) followed by the addition of 0.02 l RNAiMAX (Thermo Fisher Scientific) in 5 l Opti-MEM using a BioTek EL406 Washer Dispenser (Agilent)

At 5mg/mL concentration, an 8mg dose requires 1.6mL, which is a large subcutaneous injection

Iron overload-induced programmed cell death The academic community has consistently recognized the mechanisms related to increased mitochondrial oxidative stress and impaired energy metabolism in the context of iron overload