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Development of znO nanoparticles as an efficient zn fertilizer: using synchrotron-based techniques and laser ablation to examine elemental distribution in wheat grain

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After incubation with primary antibodies, membranes were washed three times with 0.1% Tween 20 and TBST and then incubated with horseradish-peroxidase-conjugated anti-rabbit antibody (Cell Signaling #7074) or horseradish-peroxidase- conjugated anti-mouse antibody (Cell Signaling #7076) at 1:3000 dilutions for 1 h at room temperature