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Occasional systemic symptoms including headache, nausea, dizziness or gastrointestinal discomfort in some patients

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For immunofluorescence double staining with anti-FASN and anti-8-oxo-dG, paraffin sections were incubated with rabbit anti-FASN (dilution 1:50, Cell Signaling) and mouse anti-anti-8-oxo-dG (1:250, Trevigen) at 4 C o/n in a humidified chamber