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Single cell clones were grown for immunoblot analysis and those showing loss of ATF4 protein were selected for sequence analysis involving the isolation of genomic DNA (Qiagen, 69504), PCR amplification using KOD Xtreme Hot Start DNA Polymerase (Millipore, 71975) and the primers TCGATGCTCTGTTTCGAATG and CTTCTTCCCCCTTGCCTTAC flanking the targeted deletion site, with sequencing on an ABI3730xl DNA analyzer at the DNA Resource Core of Dana-Farber/Harvard Cancer Center (funded in part by NCI Cancer Center support grant 2P30CA006516-48)

THEY ARE NOT FOR HUMAN OR ANIMAL USE

Double staining was performed: 2 g/ml Hoechst (Sigma-Aldrich, St

These advancements suggest that a combination of pharmacological, genetic, and nanotechnological approaches may offer synergistic benefits for slowing or halting disease progression (Zhou et al., 2024)

Laser hair removal is often a great option for these patients