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Results GSH depletion induces cell death in RPE cells To evaluate the effect of GSH depletion on the RPE cells, we used three different approaches: depletion of Cys 2 from cell culture media, treatment with buthionine sulphoximine (BSO, 1000 M) to block de novo synthesis of GSH, or treatment with erastin (10 M), an inhibitor of system x c 1

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By directly scavenging reactive oxygen species (ROS), it helps to prevent mitochondrial dysfunction, supports ATP synthesis, and inhibits the pathways leading to cell death, including apoptosis

Rahbar, A

169 Molecular mediators that drive organ crosstalk Interorgan crosstalk among the kidney, heart, brain, adipose tissue, liver, skeletal muscle, pancreas, and intestine plays a pivotal role in the development of insulin resistance and -cell dysfunction, which are central to the progression of diabetic complications