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ANIMAL EXPERIMENTS ESSENTIAL FOR THE UNDERSTANDING OF HUMAN INSULIN-GH-IGF AXIS PHYSIOLOGY Insulin, IGF-I, and IGF-II and Their Receptors Efstratiadis series of knock-outs of the insulin-GH-IGF-axis in mice in the early 1990s clearly confirmed its importance in fetal and postnatal growth and metabolism (21)

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Immunoblotting of the clarified lysates revealed a single band of the expected molecular mass in the transfected sample, whereas all six non-transfected extracts were devoid of discernible off-target signals (Fig