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Age and degree of GH axis decline: Patients in their late 40s and 50s typically see more dramatic early results than patients in their mid-30s starting preventively, because GH decline is more advanced and the pituitary still has enough reserve capacity to respond meaningfully to stimulation

An exonic point mutation of the androgen receptor gene in a family with complete androgen insensitivity

Prolonged hepatotoxicity with associated regenerative hyperplasia can be associated with and lead to liver tumor development (Evans and Lake 1998

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Invitrogen), 2 mM L-glutamine, 100 mM non-essential amino acid (NEAA), and 550 M 2-mercaptoethanol (2-ME