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8 (download XLSX Source data Extended Data Fig

To facilitate exchange of deuterated amides back to protons, the purified protein was incubated with 2.5 M guanidinium-HCl and 5 mM EDTA for 3 h, then dialyzed into NMR buffer (10 mM sodium phosphate, pH 7.0, 150 mM NaCl)

Similar content being viewed by others Introduction The classical way to generate temperature-sensitive alleles comprises random mutagenesis followed by large-scale screens at different temperatures

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In most organisms studied to date, these processes predominantly occur in the cytosol or in plastids of plants (Kruger and von Schaewen, 2003