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Carbamidomethylation was set as a fixed modification, whereas methionine oxidation, asparagine and glutamine deamination, and glycosylation options were set as variable modifications, according to published data ( The intact hCG analysis revealed several hCG glycoforms

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The results reveal that cell lines fall robustly into four clusters numbered C1-C4 with no appreciable gain in cluster stability when increasing to higher numbers of clusters (Figure 1A and Table S1)

That does not make them useless it means they belong under supervision, not self-prescription

Priceman, S., Tilakawardane, D., Jeang, B., Aguilar, B., Murad, J., Park, A