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After 24 h, total protein extracts were obtained from whole flies homogenized in standard lysis buffer (20 mM Tris-Cl (pH 7.5), 200 mM NaCl, 2 mM EDTA, 10% glycerol, 1% Nonidet P-40 and EDTA-free Complete Protease Inhibitor Cocktail (Roche Diagnostics))

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Fortified nutritional yeast is the most common variety and, for the purposes of this article, is what we are referring to when we talk about nutritional yeast

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For example, 50 mg may be divided into two 25 mg injections , or 30 mg may be divided into two 15 mg injections , administered on separate days or spaced within the same week depending on protocol preferences