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The sciatic nerve homogenate (10 % W/V) was then prepared using 0.1 M Tris-HCL buffer (pH 7.4) for estimation of total protein content, nitric-oxide content, reduced glutathione, and lipid peroxidation [30]

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Histamine is a chemical produced by the body to defend against allergens such as pollen, mold, and foods (KidsHealth, n.d.)

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