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Arabidopsis thaliana GSNOR-defective mutants (gsnorl-3) over-accumulate S-nitroso-thiols (SNO) in guard cells and are insensitive to ABA-induced stomatal closure (Wang et al

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The -actin protein expression was used as a loading control for each sample after stripping the membrane post-detection with buffer containing 1.5% glycine, 0.1% SDS, and 1% Tween-20 (pH = 2.2)

If Phase 2 capacity is insufficient relative to Phase 1 activity, toxin clearance is impaired and symptom burden may increase rather than resolve

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