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Brain tissue homogenates and serum samples were collected to quantify intestinal permeability markers (zonulin and occluding), oxidative-stress markers (GSH, MDA, CAT), and GABA-receptor gene expression using ELISA and biochemical assays

Membranes were then incubated for 30 min at RT in HRP-conjugated secondary antibody (Santa Cruz Biotechnology, Inc, USA) diluted in 5% milk or BSA in TBS-T

doi: 10.1126/science.1241224 230 YangY.CuiY.SangK.DongY.NiZ.MaS.et al

It is not approved for human or veterinary use, clinical administration, therapeutic applications, or diagnostic procedures of any kind

Lastly, the effects of AST appeared to be mediated through the benzodiazepine and opioid receptors, which are known to play roles in the management of neuropathic pain (Figure 11)