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J Appl Pharm Res 8(1):2943 Sharma K, Verma R, Kumar D, Nepovimova E, Kua K, Kumar A, Raghuvanshi D, Dhalaria R, Puri S (2022) Ethnomedicinal plants used for the treatment of neurodegenerative diseases in Himachal Pradesh, India in Western Himalaya

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Although the silane groups of the C18 column are also fully protonated the acidic environment provides sufficient protons (H + ) in the solution that the acidic NAC will retain its protons, improving retention on RP-HPLC

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