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ROS inactivate protein kinases that phosphorylate microtubule-associated proteins (MAPs) that maintain this dynamic equilibrium ( ROS impedes mitochondrial respiration, reducing the rate of ATP synthesis via destruction of mtDNA ( A high intracellular ratio of reduced glutathione to oxidized glutathione (GSH/GSSG ratio) is protective of microtubules in their polymerized state by the preventing oxidation of cysteine residues in tubulin subunits

Flag-Sororin was immunoprecipitated with anti-Flag M2 agarose (Sigma, A2220) for 4 h at 4 C

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