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A tissue-scale gradient of hydrogen peroxide mediates rapid wound detection in zebrafish

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The basis for each medium was a modified Sager-Granick medium: 16.2 M H 3 BO 4 , 3.5 M ZnSO 4 7H 2 O, 2.02 M MnCl 2 4H 2 O, 0.84 M CoCl 2 6H 2 O, 0.24 M CuSO 4 5H 2 O, 0.83 M (NH 4 ) 6 Mo 7 O 24 4H 2 O, 3.75 mM NH 4 NO 3 , 1.22 mM MgSO 4 7H 2 O, 0.36 mM CaCl 2 2H 2 O, 0.57 mM K 2 HPO 4 , 0.73 mM KH 2 PO 4 , 37 M FeCl 3 , supplemented with 3 mM sodium acetate and 5 mM HEPES pH 6.8