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Monitoring recommendations: Baseline investigations: Full blood count, B12 level, folate, and intrinsic factor antibodies (if pernicious anaemia suspected) During initial treatment: Potassium monitoring in severe deficiency (risk of hypokalaemia) Follow-up: Repeat full blood count after 8 weeks to confirm haematological response Long-term: Annual review to ensure compliance with maintenance injections

If your protocol requires single-use vials or doses administered within 72 hours, sterile water is acceptable

Lots of processes in the body need B12 to operate effectively

Jumping to a high dose too quickly is the most common mistake

All animal procedures were performed in adherence to the 3R principles (Replacement, Reduction, and Refinement)