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Melanocytes generate large amounts of ROS during melanin synthesis, which under normal conditions are neutralized by antioxidant systems such as glutathione and superoxide dismutase (SOD) ( 2 O 2 ) have been detected in the epidermis of vitiligo patients ( 2 O 2 induced apoptosis in PIG1 and PIG3V cells, but treatment with antioxidants could alleviate melanocyte damage ( + T cells to the skin, thereby linking innate immune activation to adaptive immunity

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The supernatant was collected into a new tube and used for protein analysis

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& Kujawa, S