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Metabolic control of autoimmunity and tissue inflammation in rheumatoid arthritis

Gong, Zhicheng

McCullough Biology, Epidemiology, and Public Health, McCullough Foundation, Dallas, Texas

Commonly reported side effects The most frequently reported side effects are mild and typically transient

Cell fractionation Cells were resuspended in fractionation buffer (250 mM sucrose, 20 mM Hepes, 10 mM KCl, 1.5 mM MgCl 2 , 1 mM EGTA, 1 mM EDTA, 1X protease inhibitor cocktail) and then sonicated (4 15 s, 50% amplitude)