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J Cell Mol Med

Simultaneously, MGS1 cells were seeded at 2 10 cells per 10 cm dish and cultured in parallel for preparation of the macrophage treatments

Dongzhu Ma, et al

There is a link among mTORC1 signaling upregulation and tumor growth, which establish that tumors could be responsive to mTORC1 inhibitors

The following monoclonal antibodies (mAbs) or polyclonal Abs (pAbs) were used in the present study: rat anti-mouse F4/80 mAb (Dainippon Pharmaceutical Company, Osaka, Japan), rabbit anti-myeloperoxidase (MPO) pAbs (Neomarkers, Fremont, CA), rabbit anti-APAP pAbs (Biogenesis Inc., Kingston, NH), rabbit anti-CYP1A2 pAbs, rabbit anti-CYP3A pAbs, rabbit anti- -actin pAbs, rabbit anti-mouse NF- B p65 pAbs (Santa Cruz Biotechnology, Inc, Santa Cruz, CA), rabbit anti-CYP2E1 pAbs (Daiichi, Pure Chemicals, Tokyo, Japan), and rabbit anti-single-strand DNA (ssDNA) pAbs (Dako, Kyoto, Japan)