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Subsequently, after 10 min incubation at room temperature in protein blocking solution (Dako, Glostrup, Denmark), sections were incubated at 4 C for 48 hours with the anti-8-Hydroxyguanosine antibody (LifeSpan Bioscience Inc, Seattle, Washington, USA)

Among these innovations, charge-reversible nanocarriers have attracted considerable attention due to their ability to overcome physiological and pathological barriers in the tumour microenvironment (TME) by altering their surface charge in response to specific stimuli, which enhances drug targeting while reducing off-target effects

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However, it is more probable that these courses of action will facilitate the evolution of bacteria into superstrains [108, 109]