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After washing, the slices were fixed with 4% PFA in PBS for 1 h at room temperature, and the fixation reaction was quenched with 100 mM NH 4 Cl in PBS for 30 min

HUBERMAN: So when people are combining different things in cocktail, it's not necessarily a bad thing, but you want to make sure that you're working with someone who's very familiar with peptides

It is known that ROS could induce both necrosis and apoptosis via activation of Caspase-3 protease, which is cleaved to a p12 subunit and a p17 subunit 38