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H&E, Alcian blue, and Prussian blue staining were performed according to the staining kits protocol (Servicebio) after deparaffinization

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Transl Gastroenterol Hepatol 6:4 Putcha D, Brickhouse M, Wolk DA, Dickerson BC (2019) Fractionating the rey auditory verbal learning test: distinct roles of large-scale cortical networks in prodromal Alzheimers disease

Consistently, a complementary strategy showed that in aged mice, where IGF-I levels are decreased, cardiac overexpression of IGF-I significantly improved cardiomyocyte contractile function[283], attenuating oxidative stress-mediated protein damage, normalizing Ca 2+ homeostasis, reversing age-related alterations in the expression of pro-apoptotic proteins, and decreasing the apoptosis rate[284]