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Experiments were performed at RT with a flow rate of 0.5 mL/min in 25 mM Tris pH 7.4, 25 mM NaCl, and 1 mM DTT

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Thus, suggesting that the low level of total GSH found in the EDTA-treated plasma stored at 4 o C is due to enzymatic degradation of total GSH rather than other biological processes [34]

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