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In brief, the pyocyanin-ascorbic acid mixture was mixed with chloroform (Univar., Ingleburn, Australia) to a dilution factor of 300 L of chloroform for every 1,000 L of the mixture in 15 mL falcon tubes and vigorously shaken manually and vortexed immediately for 5 s followed by centrifugation at 4500 g for 10 min at 4C

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Samples were analyzed in triplicate, with insulin levels reported as U/mL

At the endothelial level, activation of the PI3K/AKT/eNOS and vascular endothelial growth factor (VEGF)/fibroblast growth factor (FGF) pathways enhances nitric-oxide synthesis and angiogenic signalling, improving microvascular circulation and oxygen delivery [261,266]

Bulking Agent (Microcrystalline Cellulose), Reduced Glutathione, Capsule Shell (Hydroxypropyl Methylcellulose), Anti-Caking Agents (Silicon Dioxide & Magnesium Stearate)