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Optimization included testing the inclusion of gp42 alone or with gp350, the effect of the order of the two glycoproteins gH and gL in tandem expression constructs, the timing and dosing of repRNA delivery, the effect of soluble versus membrane-anchored antigen, and the delivery of gH/gL fused to different multimeric constructs encoded by repRNA

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Activated Wnt/-catenin further promotes the expression of Jag1 in PCECs, stimulating Notch signalling in fibroblasts and enhancing fibrosis

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In this study, we submitted mice to monthly episodes of APAP intoxication starting as a young adult (90-days-old) until an elderly age of 420 days