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For antibody titrations, the sera that bound recombinant antigens above the linear range of the assay were serially diluted (1/10, 1/100, 1/1000) in TBST, re-tested until binding fell into the linear range and the calculated arb

Despite the plethora of commercial tests, a clinically available, validated biomarker for histamine sensitivity has not yet been developed [8]

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However, the patient fears the formal diagnosis of anxiety and depression due to the social stigma surrounding mental illness