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Subsequently, the buffer of the eluted fractions was exchanged using a Vivaspin 20 Ultra centrifugal unit (30 kDa cut-off, Merck Life Science) and the proteins were further purified using a gel-filtration column (SuperdexTM 200 HR 10/300 column, GE Healthcare Life Sciences) in 20 mM Tris-HCl pH 7.4, 300 mM NaCl, 5% glycerol, and 1 mM dithiothreitol (DTT)

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Animal studies at effective doses showed no overt toxicity, but this cannot be extrapolated to human safety

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AMP-activated protein kinase activates p38 mitogen-activated protein kinase by increasing recruitment of p38 MAPK to TAB1 in the ischemic heart