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Guiyun Jin, [email protected] These authors have contributed equally to this work and share first authorship Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

doi: 10.1038/s41598-024-68966-8 135 EngelkeUFvan OutersterpREMerxJvan GeenenFAvan RooijABerdenGet al

After culture for 40 h, cells were collected and lysed in 8 ml of hypotonic buffer (10 mM HEPES pH 8.0, 10 mM KOAc, 1.5 mM Mg(OAc) 2 , 2% (v/v) glycerol, 5 mM NDSB-256 and 0.5 mM TCEP, with 1 tablet of cOmplete Mini EDTA-free Protease Inhibitor Cocktail (Roche, 11873580001) per 10 ml of buffer) by a Dounce homogenizer

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Potassium modulates electrolyte balance and blood pressure through effects on distal cell voltage and chloride