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After 48 h, biofilms were washed once with 1 PBS and treated using one of the following: 30 mM GSH or 4 g/mL amikacin individually or a combination of 30 mM GSH + 4 g/mL amikacin

We also observed that PC cells with upregulating expression of SLC7A11 at the PM level induced by VRK2 display decreased incidence of ferroptosis, which accounts for why PC cells with VRK2 high expression are insensitive to GSH inhibition alone

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These reactions can have deleterious consequences when they reverse xenobiotic metabolism