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Following a 5-min stir at ambient temperature, the solution was centrifuged at 12,000 rpm for 5 min and then rinsed with methanol to produce the CDCZ nanoparticles

Each of the fractions had a final volume of 400 L and were evaporated to dryness using a SpeedVac vacuum concentrator

In LPS and TNF--induced cell inflammation models, compared with the no treatment group, CF aqueous extract (at 0.2, 1, 5 mg mL 1 ) and cornuside (at 1, 10, 50 mol L 1 ) for 24 h significantly inhibited NF-B p65 translocation, down-regulated COX-2 and iNOS production, finally decreased PGE 2 and NO levels to control excessive inflammatory responses [36,37,38]

Collectively, to better understand the therapeutic potential of NADPH metabolism, more preclinical and clinical studies should be implemented to address these difficulties, and combined approaches with immunotherapy and/or chemotherapeutics should be pursued as the best strategies because of their synergistic effects

Data visualization and histogram generation were performed using GraphPad Prism 8.0.1 software