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Upon examination there was medial joint line and medial collateral ligament tenderness with posterior knee swelling

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Animal Experiment Luxol fast blue (LFB) staining: The sections were deparaffinized, rinsed in dimethylbenzene I, dimethylbenzene II, absolute ethyl alcohol I, absolute ethyl alcohol II, 95% ethanol, 90% ethanol, 80% ethanol, 70% ethanol, and distilled water and then incubated in an LFB solution (0.01% in 95% ethanol) overnight at 60C

doi:10.3389/fbioe.2020.629088 3