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Creatine and glycine in both serum and urine were selected as the potential biofluid biomarker panel for ESCC detection as they were the overlapping discriminative metabolites across serum, urine and tissue samples in ESCC patients, and also they were the major metabolites involved in the perturbation of glycine, serine, and threonine metabolism, the significant pathway alteration associated with ESCC progression

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doi: 10.14336/ad.2021.0225 174 CaoGLinMGuWSuZDuanYSongWet al

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5a), leading to elevated intracellular ROS and mitochondrial ROS in T cells (Supplementary Fig