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Caspase 3 is an important executioner protease in the apoptotic pathway

After washing with five column volumes of equilibration buffer, elution was performed applying a gradient over 10 column up to 500 mM imidazole

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Analysis of glutathione, glutathione disulfide, cysteine, homocysteine and other biological thiols by high-performance liquid chromatography following derivatization by n-(1-pyrenyl) maleimide