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Control Release 365 , 10891123 (2024)

Lui H, Wei X, Jang S, Jauregui M, Dong L, Balter P, Luo D, Pan T, Hunjan S, Starkschall G, Rosen I, Prado K, Liao Z, Chang J, Komaki R, Mohan R

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In various embodiments, A1 and/or A2 is a peptide comprising a solubilising group comprising an amino acid sequence comprising two or more hydrophilic amino acid residues in the peptide chain

For example, the membrane protein expression host C41(DE3) was obtained by stress screening, while the autolysin expression host BL21(DE3-lac1G) was constructed by recombining P lacUV5 with P lac sequences [10, 20, 31]