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When to use MBP: First-line choice for "difficult" proteins When solubility is the primary concern When you can tolerate the 42 kDa size during early purification When NOT to use MBP: When size matters (crystallography of small proteinsMBP may dominate crystal packing) When you need a native N-terminus (TEV leaves a Ser residue) When amylose purification is insufficient (consider adding His6) Common dual-tag strategy: His6-MBP-TEV-target

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doi: 10.1186/s12883-017-1001-4 62

These dynamics are meticulously regulated by specific proteins