elution reduced glutathione for gst-egfp fusion protein Transglutaminase 2 crosslinks the S-transferase tag, impeding protein–protein interactions of the fused In vitro GST pull-down assay.
In vitro GST pull down assay. (A) A GST fusion protein (red) is Download Scientific Diagram GFP expression in E. coli. (A) Purification workflow of GST fusion Download Scientific Diagram Research Progress Fusion Tags for Recombinant Protein Production Yuan 2025 Biotechnology and Applied Biochemistry Wiley Online Library Purification of proteins fused to glutathione S tranferase PMC GST Tag Definition & Data
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