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Drawing: After cleaning the rubber stopper with an alcohol wipe, insert your insulin syringe and draw your desired dose

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The system allowed researchers to precisely map the ~10 mV voltage-dependence shifts and calculate exact activation time constants

DNA expression plasmids were extracted and were purified from bacterial cells with NucleoBond Extra Midi Plus kit (Macherey-Nagel, Germany) and they were stored at -20C until their use for transient transfection into mammalian cells