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Behrens, E

& Dodiuk-Gad, R

Before the DOL can be determined, the following specifications must be known: (7) The molar extinction coefficient () of the unlabeled protein (for IgG = 210,000 M -1 cm -1 ) The molar extinction coefficient and absorbance maximum (A max ) of the fluorophore (for FITC = 73,000 M -1 cm -1 , A max = 516 nm) The correction factor at 280 nm (A 280 ) of the fluorophore (for FITC A 280 = 0.254) The following protocol can be used to determine the degree of labeling: Remove all excess, unbound dye by dialysis or gel filtration

doi: 10.1128/IAI.00155-09 256 RenX.EcclesD

MarkDuplicates (Picard, V.2.27.4) was used to remove PCR duplicates