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In brief, HEK293T cells (American Type Culture Collection (ATCC), CRL-3216) were cultured in complete DMEM (Gibco, 11965-092) supplemented with 10% (v/v) FBS (VWR, 97068-085) and 1% penicillinstreptomycin (VWR, 16777-164) at 37 C under 5% CO 2

Papaiakovou et al., 2024)

Although the liver can also synthesize Gln, its main function is to regulate the large amount of Gln absorbed from the intestine

Longitudinal antibody dynamics after COVID-19 vaccine boosters based on prior infection status and booster doses

Doses used clinically generally start modestly and can be adjusted upward based on the patient, with the intravenous route favored precisely because it sidesteps the oral-absorption question