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Multiple sites on SARS-CoV-2 spike protein are susceptible to proteolysis by cathepsins B, K, L, S, and V

The total cell lysate was cleared by centrifugation and supplemented with 25 L per liter culture OD 600 of BioLock avidin (IBA Lifesciences, Gttingen, Germany) to mask D-biotin from the culture medium and/or endogenous biotinylated proteins from the host cell, followed by sterile-filtration

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Substrate (e.g., glucose) consumption and oxygen consumption continue, and will actually increase, because the protons are not being pushed out against a gradient and just flow right back in without doing any work to make ATP