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Neurochem Res 50 , 56 (2025)

Cell lysates (25 to 40 g of protein) were loaded per well

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However, it is necessary to consider that the introduction of a new amide bond increases the susceptibility to hydrolysis and the topological polar surface area (TPSA), as well as contributing to the increase of hydrogen bond donor (HBD) or hydrogen bond acceptor (HBA) count