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In this paper, we present the structures of the peptide-free and peptide-bound forms of DAP BII, a member of the S46 peptidase family, which is a unique member of the clan PA peptidases

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[DOI] [PMC free article] [PubMed] [Google Scholar] 37.Pace C., Tumino D., Russo M., Le Moli R., Naselli A., Borz G., Malandrino P., Frasca F

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RNA extraction and RTqPCR Total RNA from cells was isolated via TRIzol (Takara, Otsu, Japan) and subsequently purified with chloroform/isopropanol/ethanol, and cDNA was synthesized via the PrimeScript RT reagent Kit (Takara) according to the manufacturers instructions and used as the template for qRTPCR