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La rservation devient dfinitive par le renvoi de la confirmation de rservation et sous rserve de ne prsenter aucune contre-indication la cure JoliJene

The column was washed with 150 mL of the equilibration buffer and the protein was eluted using a 400 mL gradient from 10300 mM imidazole in 50 mM KPi, 100 mM NaCl, pH 7.4

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A set of three preparations was prepared independently and measured in parallel