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An instrument with a 400-W (or higher) capacity is recommended, for example from Branson ( After low-speed centrifugation using standard preparative centrifuges (Beckman Coulter Avanti Series can be found at high-speed centrifugation is a convenient and rapid cleanup step before column chromatography (Fig

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3.3.2 Excitatory amino acid transporters Glutamate uptake is accomplished in the CNS by excitatory amino acid transporters (EAATs) that are present in the pre- and postsynaptic cell surface of neurons and astrocytes, where glutamate is preferentially recycled (163)

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According to clinical research parenteral administration (intramuscular or intravenous) is preferred over other drug administration routes in case of emergency situations as it provides first-pass metabolism avoidance, reliable therapeutic concentrations and better bioavailability of dosage (2) It can also be used in situations when oral route is not feasible