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Lumbar puncture is performed to measure the opening pressure, as well as to obtain cerebrospinal fluid (CSF) to exclude alternative diagnoses

melanogaster exposed to rotenone and TA Mitochondrial metabolic rate (cell viability) was determined based on enzymatic reduction of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) to MTT-formazan in flies at a final concentration of 5 mg/mL according to Abe and Matsuki 58

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