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Description

As expected, incubation with only reduced MESNA (75 mM) yielded no active RNase A (Figure 5)

C57BL/6N mice with SIRT5 knockout (KO) were generated by knocking out Exon 3 on SIRT5 (starts from about 12.47% of the coding region and covers 14.41% of the coding region) using CRISPR/Cas9 technology (Fig